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Chunk #7 — Materials and Methods — Animals

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Encoding of the Intent to Drink Alcohol by the Prefrontal Cortex Is Blunted in Rats with a Family History of Excessive Drinking.
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Male P rats (N = 22) were ordered from the Indiana Alcohol Research Center Animal Production Core, and male Wistar rats (N = 21) were ordered from Envigo. All animals were shipped via truck to our vivarium, and were single housed and placed on a 12/12 h dark/light reverse cycle. Animals were ≈70 d of age before testing and had ad libitum access to food and water. All procedures were approved by the Animal Care and Use Committee and conformed to the Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research (National Academic Press, 2003).