DSM4 criteria for alcohol dependence classification as provided by the New South Wales Tissue Resource Centre at the University of Sydney. For each individual in the RNA-Seq dataset a module eigen value was calculated for each module. This module eigen value was used to perform the correlation analysis of the traits (e.g. alcohol dependence, alcohol consumption and Audit scores) with each whole module. Digital deconvolution showed no significant differences in the percentage of neurons, astrocytes and microglia in the PFC of alcoholics and controls (Supplementary Figure 3)21; therefore we did not perform any correction for cell-type heterogeneity. Assigned modules were functionally annotated against known molecular/functional categories and pathways using Ingenuity Pathway Analysis (IPA).
