Single colonies of HT115 bacteria containing cloned L4440 plasmids with fragments corresponding to unc-37, hlh-2, mei-1 or rba-2 were treated as described in the optimal induction method above, except that for each gene, NGM plates were used with the following IPTG concentrations: 0, 1 pM, 1 nM, 1 μM, 1 mM, and 10 mM. Worms were incubated for 72 h at 15°C before being replica plated and scored.
