Frontal cortex sections (400 μm) were flash frozen using liquid nitrogen, minced and resuspended in 4 mL, then passed through a 20 μm then a 5 μm filter before being centrifuged for 10 minutes at 500 g. The pellet was then resuspended in 1 mL of “Dissociating Buffer” (see above). Isolated nuclei were Hoechst stained and counted, then analyzed in standard Drop-seq devices at a concentration of 176 nuclei/μL.
