Using air treatment as a control, we compared the top 200 most significantly differentially expressed (lowest P-values) transcripts from air vs. odors (Figure 2Ai), air vs. EtOH (Figure 2Aii), and air vs. trained (Figure 2Aiii). There was surprisingly little intersectional overlap in specific transcripts between condition comparisons, including between air vs. EtOH and air vs. trained (Figure 2B). This indicated that experiencing odors, ethanol intoxication, or making ethanol-odor memories produced distinct, molecularly separable changes in the lasting transcriptional state of MB nuclei. Using hypergeometric statistics, the number of overlapping transcripts was found to be statistically greater than would be expected by chance alone (Figure S4B). The extent of intersection was visualized with an upgraded Venn diagram plot generated by an R package called “UpSetR” (Figure 2B, Figure S4B). Overlapping top P-value isoforms and their predicted human orthologs between condition comparisons are listed in Figure 2C.
