In the analysis of stratified heritability enrichment using LDSC28 (see Methods), several cell line functional enrichments were significant (FDR < 0.05) for AUDIT-C (Supplementary Data 19) and AUD (Supplementary Data 20). Cell type group partitioning heritability enrichment analyses indicated that central nervous system (CNS) was the most significant cell type for AUDIT-C (Fig. 2b, upper panel; Supplementary Data 21) and the only significant cell type for AUD (Fig. 2b, bottom panel; Supplementary Data 22). Enrichments for AUDIT-C were also detected for cardiovascular, adrenal or pancreatic, skeletal muscle, other, and liver cell types in descending order of significance. We also tested the heritability enrichments using data from gene expression and chromatin to identity disease-related tissues or cell types29 (Supplementary Data 23–32). We found a few epigenetic features in brain tissues—e.g., H3K4me1, H3K4me3, and DNase—that were significantly enriched for each trait.
