Chunk #30 — RESULTS — Differential expression of signaling proteins in calcium, cyclic nucleotide and small GTPase 2nd messenger pathways in PCPs — cGMP signaling modules in LPC and CHC
are nearly absent in SST/CR and VIP cells (Figure 5E). This suggests that cGMP signaling is prominent in the former three cell types but weak in the latter three populations. Consistently, cGMP-degrading Pde1a, 5a, 11a are also highly enriched in LPCs and CHCs (Figure 5E), which may regulate the spatiotemporal dynamics of cGMP in these cells. Among the two types of cGMP-dependent PKGs, Prkg1 is found in all PCPs but with major enrichment in CHCs (Figure 5E). Furthermore, several PKG-regulated ion channels are also differentially enriched in these two cell types (Figure 5F, Figure S5B). The stunning coordination in the expression of multiple (8–9) genes encoding almost the entire NO-cGMP pathway in LPCs and CHCs, from ligand synthesis and 2nd messenger signaling to potential effectors, suggests orchestration by a gene regulatory network.
