Chunk #32 — Experimental Procedures — Immunostaining and FACS Analysis

Source

In Vitro Modeling of Blood-Brain Barrier with Human iPSC-Derived Endothelial Cells, Pericytes, Neurons, and Astrocytes via Notch Signaling.

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1:1,000), anti-SM22α antibody (ab10135; Abcam; 1:500), anti-CALPONIN (ab46794; Abcam; 1:500), anti-NG2 (14-6504-80; eBioscience; 1:500), anti-NESTIN antibody (MAB5326; Millipore; 1:500), anti-mouse TUJ1 antibody (MAB1637; Millipore; 1:1,000), anti-rabbit TUJ1 antibody (845501; Covance; 1:1,000), anti-GFAP antibody (IS524; Dako; 1:100), anti-BCRP antibody (MAB995; R&D; 1:200), anti-PGP antibody (MA1-26528; Thermo Fisher; 1:200), anti-NICD antibody (ab52301; Abcam; 1:300), anti-CLAUDIN5 antibody (352500; Life Technologies; 1:500), and anti-ZO1 antibody (339100; Novex; 1:500).