Fluorescent SureFISH probes were purchased from Agilent Technologies (SureFISH 11q22.3 ATM 153kb and SureFISH Chr11 CEP 798kb). iPSC were spread on positively charged glass slides using protocol reported previously (Moralli et al., 2011). FISH was performed following Agilent protocol. Probes were visualized by a Leica DM5000B fluorescence microscope. Probe signals were enumerated in 200 cells for each iPSC subline.
