Animals that were compared were tested on the same plates under identical conditions. Untreated and treated raw speeds are shown in Table S1. Relative speeds (treated/untreated X 100) for ethanol effects were calculated and used for all strains in statistical comparisons. Statistics were performed using Prism 5.0 (GraphPad). Comparisons of 10-minute treated speed, comparisons of recovered speed (treated speed at 30 minutes minus treated speed at 10 minutes), and comparison of untreated speed on NGM versus cholesterol-depleted media were made using 1-way ANOVA, with Tukey’s multiple comparison posthoc tests. Development of AFT was tested by unpaired two-tailed t-test.
