The inter-platform GC-bias comparisons on human and microbial samples presented above are broadly compatible with previously published work [3,5]. However, we clearly observed more bias between 60% and 70% GC on R. sphaeroides in Ion Torrent data than on MiSeq data, while Liu et al. [7] found the reverse when comparing Ion Torrent to HiSeq. Our R. sphaeroides results are compatible with the results reported by Ion Torrent for the high-GC Rhodopseudomonas palustris genome [28].
