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Chunk #33 — Methods — Libraries preparation

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Joint mouse-human phenome-wide association to test gene function and disease risk.
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yes

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Six libraries were constructed. Three mate-paired libraries with insert sizes of 1, 2 and 3 kb were prepared for ligation-based sequencing (ABI SOLiD 2 and 3). Three paired-end libraries with insert sizes between 175 and 215 bp were prepared for sequencing by synthesis (Illumina GAII and HiSeq 2000). Standard protocols recommended by the manufacturers were used in all cases.