In summary, we provide the first demonstration that gene specific RNA-seq can detect and quantify unexpected rare splice junctions, and we show novel splicing complexity of the GABAB1 gene suggesting that this gene is much longer than previously known. We introduce two new strategies for comprehensive analysis of gene expression and splice junctions and report novel GABAB1 splicing perturbation following chronic alcohol in human brain. RNA-seq provides a powerful tool for divulging the role of gene expression and splicing variants in alcoholism and other psychiatric diseases.
