(Figure 3A–C, lower panels), a marker of tangentially-migrating cortical interneuron precursors (Anderson et al., 1997). Importantly, western blot analysis at day 32 showed that LHX6 protein, a marker of post-mitotic, migratory cortical interneuron precursors in the mouse, was selectively enriched in the 10-18 condition. In contrast, the hypothalamic marker RAX was selectively enriched in the 2-18 condition (Figure 3D). Additional immunocytochemical data showed expression of DLX2 and ASCL1 in most neuronal progeny derived from the day 10-18 NKX2.1::GFP+ progenitors (Figure 3E–G). These data indicate that following withdrawal of extrinsic SHH activators, NKX2.1::GFP+ progenitors give rise to region-specific postmitotic neurons including immature cortical interneurons.
