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Chunk #6 — 2. Material and methods — 2.1. Passaging and maintenance of NPCs — 2.1.1. Thawing NPCs

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.
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NPCs were derived from hiPSCs as previously described [33]; NPCs can be stored indefinitely at −180 °C in NPC media supplemented with 10% DMSO.