TE-1 cells were grown to confluence in six-well culture plates and starved in medium supplemented with 0.1% serum for 48 h. Confluent cell monolayers were scratched and cells were washed twice followed by the addition of fresh medium supplemented with 0.1% FBS in the absence or presence of 50 ng/ml of HGF or, alternatively, 1 or 10 ng/ml of FGF2 (HGF and FGF2: Sigma H1404 and F0291, respectively). Closure of the scratch was monitored using a 4× objective at 0, 24, 48, and 72 h. Digital pictures were analyzed using AutoCad software, and scratched cell-free areas were calculated.
