Chunk #34 — METHODS — shRNA Design Rules

Source

lincRNAs act in the circuitry controlling pluripotency and differentiation.

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yes

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For each lincRNA we designed 5 hairpins by extending the previously described design rules22 accounting for the sequence content of the hairpin, miRNA seed matches, uniqueness to the target compared to the transcriptome and the genome, and number of lincRNA isoforms covered.