are more likely to have samples with failed sequencing reactions.8 At the outset of the project, we also tested RNAs of different quality (RIN 2.0–9.0) across multiple different library construction protocols for RNA sequencing. The most scalable and robust library construction protocol was the Illumina TruSeq protocol with Poly-A selection, which performed best with more intact RNA with RIN values of 6.0 or higher.
