t(7)=5.62, p=0.0008; ClicEY04209 females, t(8)=13.04, p<0.0001, n=3–5). (Note that data in Figure 3 and Supplemental Figure S3 are T50 values that reflect the time required for ethanol to inhibit negative geotaxis by 50%. Higher values indicate blunted ethanol sensitivity; i.e. higher doses of ethanol are required to produce the same level of response.) In contrast to the effect of Clic mutations on ethanol sensitivity, we did not observe a significant alteration in rapid ethanol tolerance in these animals (Supplemental Figure S4; two-sample t tests; ClicG0472/+, t(16)=0.777, p=0.448; ClicEY04209/+, t(17)=1.86, p=0.080; n=9–10). Clic might therefore be an important determinant of ethanol sensitivity in Drosophila without having major effects on rapid ethanol tolerance in this species.
