30% and 40% iodixanol layers. Nuclear concentration and prep quality were ascertained by loading on a hemocytometer and were diluted to a concentration of 80–100 K and 15% iodixanol with Buffer HB prior to loading on InDrops v3 platform. Single-nuclei suspensions were encapsulated into droplets, lysed, and the RNA within each droplet was reverse-transcribed using a unique nucleotide barcode127. Approximately 6000 nuclei, in two batches of 3000 nuclei each were processed per library and sequenced on Illumina NovaSeq S2 chips (at a density of approximately 20,000 reads/nucleus).
