ES cells were infected and lysed at day 4 with 150ul of Qiagen’s RLT buffer and 3 replicates of each virus plate were pooled for RNA extraction using Qiagen’s RNeasy 96-well columns (74181). RNA extraction was completed following Qiagen’s RNeasy 96-well protocol with the following modifications: 450ul of 70% ethanol was added to 450ul total lysate prior to the first spin. An additional RPE wash was added to the protocol, for a total of 3 RPE washes.
