Third, the study incorporated a user-defined template for normalization within the DTI analyses. Although this procedure is one of several recommended for use with TBSS, the pattern of findings may have differed had we used the standard FMRIB58_FA standard-space image or had we created a template based on only the 55 participants in the current study. The current strategy reduces potential normalization errors through the use of an age-appropriate template and by eliminating the potential for nuisance variance in comparing findings across studies (see 66 for an example) published from our laboratory.
