All procedures were performed under an IUCAC approved protocol. Primary cortical and hippocampal neuron cultures were derived from embryonic rat (E18) as previously described (Loo et al., 1993). Briefly, dissected tissue was dissociated with trypsin, triturated, and plated on 6-well plates coated with poly-L-lysine coated in NB medium (serum-free Neurobasal supplemented with 1% B27). Cells were plated at a density of 5 × 106 cells/ml and cells were fed once a week with 50% media change until used for assays.
