Following the same logic behind our examination of cohesion, we characterized the pattern of LOH events observed in young cells when sir2 was deleted. We found that elevated MET15 LOH rates in young sir2Δ cells were dependent on FOB1 (Figure 7A). In this regard it shows similarity to the age-associated LOH phenotype. However, the ratios of reciprocal to non-reciprocal events in sir2Δ cells was significantly shifted to favor non-reciprocal events (Figure 7B), indicating that loss of Sir2 function does not accurately phenocopy the age-associated LOH phenotype.
