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Chunk #27 — METHODS — Methylation analysis

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A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs.
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To count differentially-methylated promoters that supported variance due to cellular origin or genetic background, within-sample methylation difference was compared to the between-sample methylation difference for each promoter in sets based on cellular origin (hESC/hiPSC) and cell background (HUES2/HUES3). The promoter was assigned to the set with the lesser methylation difference, such that promoters in the hESC/hiPSC set showed greater methylation difference between hESCs and hiPSCs and lesser metylation difference between HUES2 and HUES3.