washed three times in PBS and then mounted with Mounting medium. For Immunocytochemistry, Cultured cells were briefly fixed with a 4% PFA/PBS solution for 10 min at RT, permeabilized and blocked by the pre-cold buffer (0.4% Triton X-100 and 3% BSA in PBS) at 4 °C. Coverslips were covered with diluted primary antibodies overnight at 4 °C. Secondary antibodies and DAPI were incubated for 1 h at RT. Immunofluorescence images were taken using Leica SP8 and Zeiss LSM 780 confocal laser microscope.
