Chunk #24 — PU.1 induces sequential nucleosome remodelling and histone H3K4 monomethylation

Source

Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities.

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set of induced sites, analogous to that suggested by the Group II H3K4me1-marked nucleosomes, resulting in further expansion of the linker region centered on the PU.1 binding site and compression of nucleosomes in either direction along the DNA for approximately 1 kb. In concert, these findings suggest a sequence of events at Group I sites in which PU.1 binding induces nucleosome remodelling, which is followed by monomethylation of H3K4.