Association with BMI was tested as in stage 1, assuming an additive model. Logistic regression analysis was used to test for association with the risk of being overweight (defined as BMI ≥ 25 kg/m2) or obese (BMI ≥ 30 kg/m2), with adjustment for age, age2, and sex, testing for SNP effects in an additive genetic model. Evidence for association between our replicating SNPs and type 2 diabetes15, lipidlevels16 and coronary artery disease17,18 was extracted from publicly available datasets. The effect of the replicating SNPs on expression of nearby genes was determined from publicly available eQTL GWA studies from lymphocytes42 and brain tissue27.
