GluA1−/− mouse line was generated as described [10]. GluA1 line was backcrossed to C57BL/6J mouse line (Harlan BV., Horst, The Netherlands) at least 7 times prior to use. For the experiments, the GluA1−/− mice and the GluA1+/+ littermate controls were from heterozygous breedings. The animals were genotyped using PCR from tail-tip biopsy samples as described [10]. In the behavioral experiments, the animals were housed 2–4 animals to a cage from weaning, until used at the age of 2.5–4 months. All animals were naïve to pharmacological and handling manipulations until the start of the experiments. In the electrophysiological experiments, the animals were used at the time after weaning, at the age of 20–26 days. The animal facility had lights on from 6 a.m. until 6 p.m., temperature set at 20±1°C and relative humidity at 50±1%. Water and standard rodent food pellets (Harlan) were available ad libitum. All the experiments were performed during the light phase between 8 a.m. and 13 a.m. Altogether 156 GluA1+/+ (76 females, 80 males) and 129 GluA1−/− (60 females, 69 males) animals were used.
