Zeisel et al., 2015), but their correlations to bona-fide biological types jointly defined by anatomical and physiological features remain unclear. Thus although scRNAseq allows comprehensive and quantitative measurements of gene expression, a fundamental unresolved issue is how transcription profiles might contribute to the molecular genetic basis of neuron types. Discovering the transcriptional basis of neuronal identity is prerequisite to deciphering neuronal diversity and enumerating cell census. Furthermore, high-resolution transcriptional portraits that mechanistically explain and predict the multi-faceted yet functionally congruent cell phenotypes have yet to be achieved.
