Full-length human DISC1 cDNA was amplified by PCR and subcloned with HA-tag through AgeI and EcoRV into the pFUGW vector. The 4-bp deletion mutation was introduced through a synthesized long length PCR primer and cloned with Flag-tag through AgeI and EcoRV into the pFUGW vector. All expression plasmids were confirmed by DNA sequencing.
