Chunk #13 — Materials and methods — RNA-seq analysis of miRNA and mRNA transcriptomic changes in eight brain regions of AUD subjects (192 Set 1 RNA samples) — Microarray analysis of miRNA and mRNA transcriptomic changes in six brain regions of AUD subjects (96 Set 2 RNA samples)
Console (EC) software (v1.4.1) with the “MicroRNA Arrays – RMA (robust multi-array average) + DABG (detection above Background)-Human only” workflow as the default analysis for background adjustment and signal normalization as well as log2 transformation to create probe-level summarization files (or CHP files). Quality control (QC) analysis of the CHP files was performed within the EC software, and the quality of the miRNA expression array data was visualized using box plots (Fig. S2a). The CHP files for case and control samples were further analyzed by statistical programs to identify differentially expressed miRNAs and other small noncoding RNAs. The Affymetrix miRNA expression data has been deposited in the NCBI GEO database (accession number: GSE180700).
