We identified two ADHs in worms using a method described by Williamson et al. (1991), in which we exploited the fact that ADH metabolizes allyl-alcohol to the toxin acrolein. Inactivation of either sodh-1 or H24K24.3 them conferred resistance to allyl-alcohol – induced lethality. We inactivated these two genes, both singly and in combination, in our analysis of ADH pathway function in the behavioral responses to ethanol.
