Chunk #38 — Methods — Processing and alignment of MethylC-Seq data to identify methylated cytosines

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Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells.

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For the previously published HSF123 and H9-Laurent24 data sets, the GEO sequence read data were mapped using the MethylC-Seq pipeline (H9-Laurent) and BS Seeker (HSF1)36 (settings: -e ≤ 55, -m 3), and post-processing and methylcytosine identification was performed using MethylC-Seq pipeline as described earlier.