During the GnRH stimulation test, serum levels of luteinizing hormone and FSH were measured 15 minutes before the intravenous administration of 100 μg of GnRH, at the time of administration, and 15, 30, 45, and 60 minutes after administration. In both sexes, basal luteinizing hormone levels higher than 0.15 U per liter were considered to be pubertal levels, and peak GnRH-stimulated luteinizing hormone levels higher than 5.0 U per liter were considered to be pubertal responses.22 Basal estradiol levels higher than 15 pg per milliliter and basal testosterone levels higher than 12 ng per deciliter were considered to be pubertal levels.
