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Chunk #31 — Methods — Genetic mapping

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The Netrin-1 receptor DCC is a regulator of maladaptive responses to chronic morphine administration.
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Whole-genome computational genetic mapping- Whole-genome variation information was obtained from public data bases (10 inbred strains) [51] and through next-generation sequencing of commercially obtained DNA (The Jackson Laboratory) for the remaining 13 strains (Beijing Genomics Institute, BGI) [52]. Haplotype-based computational genetic mapping (HBCGM) was conducted as described previously [53], and used to identify those genomic regions whose genetic variation pattern was correlated with input phenotypic values for the 23 inbred strains utilized in these studies.