pAAV-hSyn-hM3Dq(Gq)-mCherry was a gift from Bryan Roth (Addgene viral prep # 50474-AAV8) and pGP-CMV-GCaMP6f was a gift from Dr. Kelvin Kwan. To clone the hSyn-hM3Dq-mCherry and hSyn-GCaMP6f lentiviral constructs used in this study, the coding sequences were amplified from these templates by PCR (Primestar Takara) and incorporated into a lentiviral backbone vector under the human synapsin promoter (hSyn) using isothermal assembly.24 The primers used were as follows:
