Chunk #25 — Results — Kir3.2 protein levels are increased by increased Kcnj6 dose

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Evidence that increased Kcnj6 gene dose is necessary for deficits in behavior and dentate gyrus synaptic plasticity in the Ts65Dn mouse model of Down syndrome.

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samples showed no difference in the protein levels in these experimental groups (Kir3.1: F1,6 = 0.09, p = 0.46; Kir3.2: F1,9 = 0.01, p = 0.49) (Fig. 2B). Comparison of Ts65Dn:Kcnj6++− vs. Ts65Dn:Kcnj6+++ samples again showed a reduction of Kir3.2 to the levels observed in 2N:Kcnj6++ samples (F1,9 = 3.3, p = 0.005) and no change in the levels of Kir3.1 (F1,7 = 0.51, p = 0.31) (Fig. 2C). Thus, in Ts65Dn mice, Kcnj6 gene dose regulated the levels of Kir3.2 but not Kir3.1.