Using the protocol of Smith and Weiss (1999), 10 rats in each strain were injected i.p. with saline and 10 rats in each strain were injected with 1 g/kg ethanol once each day for 5 consecutive days; 24 hr after the last injection, rats were killed by decapitation, the brains quickly removed and frozen in isopentane in dry ice. Brains were stored at -70° C until sectioned. Two samples were lost during the sample analysis, i.e., one saline injected P rat and one ethanol injected W rat. Therefore, there were 10 samples in 4 of the 6 groups, and 9 samples in the remaining 2 groups.
