The colony of cyp2a5−/− mice was created by crossing male C57BL/6 cyp2a5−/− mice (ref. 22) (kindly provided by Dr. Xinxin Ding, SUNY College of Nanoscale Science and Engineering, Albany, NY, USA) and female C57BL/6 wild type mice (purchased from Charles River Laboratory, MA, USA). Littermates (cyp2a5+/+) were bred as a colony of WT control. The Pparα−/− mice were purchased from Jackson Laboratory (Strain #008154). The colony of Pparα and Cyp2a5 double knockout mice (P-A- mice) was created by crossing the Pparα−/− mice with Cyp2a5−/− mice. Littermates not expressing Cyp2a5 but expressing Pparα (P+A-) and expressing Cyp2a5 but not expressing Pparα (P-A+) were used as control mice. All the mice were housed in temperature-controlled animal facilities with 12-hour light/12-hour dark cycles and were permitted consumption of tap water and Purina standard chow ad libitum. The mice received humane care, and experiments were carried out according to the criteria outlined in the Guide for the Care and Use of Laboratory Animals. The animal studies were approved by Animal Care and Use Committee at Icahn School of Medicine at Mount Sinai and University Committee on Animal Care at ETSU, respectively.
