Every 12th section was processed for Fluoro-Jade B (FJB), as described (Obernier et al., 2002; Schmued and Hopkins, 2000). Sections were mounted on Superfrost Plus® slides (Fisher Scientific) and dried overnight. Slides were processed through 1% NaOH in 80% EtOH, 70% EtOH, dH2O and then 0.06% KMnO4 for 10 min. After a wash in dH2O, slides were incubated in the dark in FJB (Chemicon) for 20 minutes. Following washes in dH2O, sections were dried and coverslipped in Cytoseal® (Stephens Scientific).
