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Chunk #13 — Materials and methods — Test procedures

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Positive relationship between dietary fat, ethanol intake, triglycerides, and hypothalamic peptides: counteraction by lipid-lowering drugs.
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Experiment 4 tested the effects of gemfibrozil on OX expression. Rats (n = 30) were trained to drink 7% ethanol and were acclimated to the gavage procedure as in Experiment 3. Subjects were tested in a between-subject design, wherein rats were administered gemfibrozil (n = 15) or vehicle (n = 15) for 5 d. On the 5th day, after they had received their last gavage, ethanol and food intake were measured for 1 h, and rats were then sacrificed for analysis of OX mRNA using real-time quantitative PCR. Trunk blood was collected at the time of sacrifice for measurement of TG levels.