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Chunk #4 — MATERIALS AND METHODS — SNP GENOTYPING

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The dopamine receptor D2 (DRD2) SNP rs1076560 is associated with opioid addiction.
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yes

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SNP genotyping was performed using Taqman® SNP Genotyping Assays (Applied Biosystems Inc. (ABI); Foster City, CA, USA) as per manufacturer protocol. Quality control was maintained by genotyping 10% duplicates which were checked for genotype concordance across the populations. Duplicate concordance was 100% across all populations genotyped. Genotypes were checked for deviation from Hardy-Weinberg Equilibrium (HWE) as a second measure of quality control. No significant deviations from HWE were observed in cases or controls in either population (all tests p>0.51).