SNPs in both LD blocks 1 and 2, but is flanked by rare SNPs. Following preamplification using the method of Zhang et al. (Zhang et al., 1992), TaqMan assays (ABI) were performed according to the manufacturer's instructions in a 384 well format using a Biomek 3000 Laboratory Automation Workstation (Beckman Coulter) for sample preparation, an Applied Biosystems 9700 thermocycler for temperature cycling, and an Applied Biosystems 7900 PRISM Genetic Analyzer for genotype calling with the 7900HT Sequence Detection System software version 2.3 (Applied Biosystems).
