fate emerges during the differentiation process. Single-cell sequencing methods provide powerful resolution into the heterogeneity of directed differentiation culture systems (Biddy et al., 2018, 2018, 2018; Camp et al., 2018; Karow et al., 2018). Previously, we have used single-cell mRNA sequencing (scRNA-seq) to dissect the differentiation path from mouse embryonic fibroblasts and human pericytes to neurons and identified previously undescribed heterogeneity generated by the overexpression of the pioneer factor ASCL1 (Karow et al., 2018; Treutlein et al., 2016). Here, we set out to characterize NGN2-iNeuron heterogeneity, identify the cell states that are generated during differentiation, and analyze the dynamics of the differentiation process using scRNA-seq.
