To investigate the functional role of the MOR N40D variant in a human neuronal context, we obtained iPS cells from multiple individuals of European descent carrying homozygous alleles for either MOR N40 (n=4) or MOR D40 (n=3) (Supplemental Fig. 1A). The rs1799971 genotype and the pluripotency of all seven iPS cell lines were confirmed by sequencing and colocalized immunocytochemistry (ICC) for OCT4 and Tra-1-60 (Fig. 1A–B).
