RNAs were extracted from the cerebellum cortex of 132 samples using the RNeasy Mini kit (Qiagen, Valencia, CA). The concentration and A260/A280 ratio were measured on the NanoDrop spectrophotometer. The ratio of 28S to 18S rRNA and RNA Integrity Number (RIN) were measured using an RNA LabChip kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). In order to avoid use of seriously degraded samples, only RNA samples with a RIN > 7 were used for the expression profiling. Affymetrix Human Gene 1.0 ST array was used for whole genome transcriptome profiling at the NIH Neuroscience Microarray Consortium at the facility at Yale University.
