Chunk #53 — Methods — Flow cytometry analysis of Csf1r−/− and PLX-3397-treated animals

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Neurotoxic reactive astrocytes are induced by activated microglia.

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Both Csf1r−/− and PLX-3397 treated animals (and appropriate controls: Csf1r+/+, control chow-treated animals) received an i.p. injection of LPS (5 mg/kg). Twenty-four hours after LPS injection, animals were killed and brains prepared for downstream processing (see below).