GST fusion constructs. The p270 fusion protein experiments were originally performed with the product of plasmid pNDX (9). For the mutagenesis studies, a shorter expression construct designated pNDB8 was generated to be more comparable in size with the Dri fusion peptide. The NDB8 plasmid expresses amino acids 958–1188 of p270 (numbering is according to the sequence at accession number NP_006006). The Dri fusion protein is the product of p410 (4), which was kindly provided by R. Saint (University of Adelaide, Australia) and expresses Dri residues 258–410 (according to accession number AAB05771).
