For analysis of cell proliferation in the dentate gyrus, adult mice at the end of 14 day treatment of SB-216763 or saline were injected with BrdU (200 mg/kg body weight, i.p.) and sacrificed 2 hrs later. Coronal brain sections (40 μm thick) were prepared and processed for immunostaining using anti-BrdU antibodies (rat; 1:400; Accurate). Images were acquired on a Zeiss LSM 510 Live confocal system (Carl Zeiss). Stereological quantification of BrdU+ cells within the subgranular zone and granule cell layer were carried out as previously described (Ma et al., 2009).
